RNA SEQUENCING OF SQUAMOUS ESOPHAGEAL CANCER:FROM THE COLLECTION OF THE BIOMATERIALUNTIL SEQUENCING ON HISEQ2000
DOI:
https://doi.org/10.34689/wrt1h996Keywords:
RNA sequencing , transcriptome , squamous esophageal cancerAbstract
The squamous esophageal cancer is a disease which is highly frequent in Kazakhstan, the
frequency of the disease is 10,1:100 000. In the mortality structure esophageal cancer takes place
among men the 5th place, among women the 9th place in the Republic of Kazakhstan. The molecular mechanisms which contribute to the initiation and progression of the cancer pathology are not
investigated enough. Nevertheless, the lack of the sensitive and specific biomarkers for the diagnosis of
the cancer diseases, underlines about the necessity of the additional research works to study about the
development of carcinogenesis. Investigation of the transcriptome profile of tumor cells is perspective in
this aspect of the study.
The paper describes the stages of the sample preparation and sequencing of RNA, based on a new
generation sequencing technology. Each step from the moment of the sampling of biological material,
isolation of total RNA, preparation of libraries have an impact on obtaining of the high-quality data in
subsequent sequencing.
References
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Рахимова С.Е., Каиров У.Е., Ережепов Д.А., Молкенов А.Б., Жалбинова М.Р., Абильмажинова А.Т.,
Жуков Ю.В., Омаров М.Х., Акильжанова А.Р. Өңештің жалпақклеткалық ісігі кезіндегі РНҚ секвенирлеу:
биоматериалды жинаудан HiSeq2000 жүйесінде секвенирлеуге дейін / / Ғылым және Денсаулық сақтау.
2016. №5. Б. 53-63.
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Copyright (c) 2026 Сауле Рахимова, Улыкбек Каиров, Даурен Ережепов, Асхат Молкенов, Мадина Жалбинова, Алия Абильмажинова, Юрий Жуков, Марат Омаров, Айнур Акильжанова (Автор)
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