DEVELOPMENT OF HALOPLEX PANEL AND PREPARATIONOF DNA LIBRARIES FOR TARGETED SEQUENCINGOF CARDIAC ARRHYTHMIAS
DOI:
https://doi.org/10.34689/52bqmv03Keywords:
HaloPlex cardiogenetic panel , targeted sequencing , cardiac arrhythmiasAbstract
Method of preparation of custom HaloPlex cardiogenetic panel (Agilent Technologies) and method of
preparation of DNA libraries for targeted sequencing of 96 genes associated with cardiac arrhythmias
were described in details in this article. SureDesign Online Design software (Agilent Technologies) was
used to create the HaloPlex panel. Final design was developed for Illumina platform using Human
Genome version 19, GRCh 37. 19958 amplicons were generated by the program, 99,46% of all target
regions were covered successfully. DNA libraries were prepared for 48 samples using the custom
HaloPlex cardiogenetic panel.
References
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Akhmetova A., Abilova Zh., Bekbosynova M., Panzitt K., Trajanoski S., Guelly C., Akilzhanova A. Development
of HaloPlex panel and preparation of DNA libraries for targeted sequencing of cardiac arrhythmias. Nauka i
Zdravookhranenie [Science & Healthcare]. 2016, 5, pp. 43-52.
Ахметова А.Ж., Абилова Ж.М., Бекбосынова М.С., Panzitt K., Trajanoski S., Guelly C., Акильжанова А.Р.
HaloPlex панелін әзірлеу және жүрек аритмияларын таргетті секвенирлеуге ДНҚ-кітапханаларын
дайындау / / Ғылым және Денсаулық сақтау. 2016. №5. Б. 43-52.
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Copyright (c) 2026 Айнур Ахметова, Жаннур Абилова, Махаббат Бекбосынова, Katrin Panzitt, Slave Trajanoski, Christian Guelly, Айнур Акильжанова (Автор)
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