TOLMEN`S LABYRINTH FOR THE DEVELOPMENT OF ANEXPERIMENTAL MODEL OF BRAIN PATHOLOGY IN MICEIN THE STUDY OF POLYMER MUCOADHESIVE DOSAGE FORMS

Abstract

Introduction: A promising direction for improving bioavailability in brain tissues is the use of water-soluble polymers for
intranasal use. Due to the direct communication through the lattice bone of the nasal cavity with the brain, it became
necessary to study water-soluble polymers on the nasal mucosa.
Objective: To conduct a study of modeling brain pathology for the study of polymer mucoadhesive dosage forms in mice
by round-the-clock monitoring of behavior in a maze.
Methods: Violations of cognitive functions of the brain in experimental groups were studied on experimental mice: with
passage through a maze with preliminary training, passage through a maze after the use of inhaled anesthesia of Isoflurane.
Results: The severity of CNS disorders in the two experimental groups with the use of Isoflurane was different. After
waking up, all mice in the control group showed a decrease in motor activity, an increase in the time to overcome the maze
and a directly proportional dependence of the duration of sleep on the dose of the injected Isoflurane solution. The weight of
experimental mice affects the time of passage through the maze in 100% of cases. The dose of toxic effects of Isoflurane on
mice corresponding to 17 drops has been established. Analysis of all data showed in the experimental group of mice in 90%
of cases, with an increase in the dose of Isoflurane, the time to overcome the maze increased exponentially. In 2 cases
(10%) after waking up in a maze, there was no decrease in the motor activity of mice. The experimental group with a
preliminary acquaintance of the maze had a picture different from the one in which there was no acquaintance. Despite the
rapid adaptation of mice in the environment, during the first 2-3 minutes the mice moved slowly. Compared with the group
without prior acquaintance, a decrease in motor activity in the new conditions after the use of Isoflurane and the duration of
the time of overcoming through the maze was noted in 80% of cases and the average time was 980 seconds, whereas in the
experimental group with preliminary acquaintance this value was 193 seconds.
Conclusions: Tolman's labyrinth allows us to assess the level of cognitive impairment of the brain in mice and the time
to overcome it to serve as a basic criterion. The body weight of mice influences the time of passage through the maze
(p=0.008).